This session will provide an overview of the original core 13 loci used in the federal DNA database (NDIS), discuss the FBI's recent expansion to a "core 20 loci," and the new "megaplex" kits designed to test for more loci. Today there are over 12 million "offender profiles" in NDIS, a far greater number than when the FBI created NDIS 20 years ago. Due to this exponential growth, forensic DNA researchers sought a solution for both "adventitious matches" in the database--false matches between people with similar DNA profiles--and distinguishing the profiles of close relatives. Ultimately, the FBI expanded its "core loci" and mandated that state labs participating in CODIS validate "megaplex" kits that can test for the full 20 loci. As of January 1, 2017, all CODIS laboratories should have these new DNA test kits online and available for casework. This session will discuss these changes, pros and cons of the new kits and testing for more loci, and host a Q&A with a forensic DNA scientist.